Restriction-ligation-independent production of a TVCV infectious clone and a TVCV-based gene expression vector

Transgenic expression of proteins in plants is central to research and biotechnology, and, often, it desirable obtain this without altering the nuclear or plastid genomes. Thus, vectors based on plant viruses that infect multiple cells are useful; furthermore, they also advantageous for studies life cycle virus itself. Here, we report development an vector a Turnip vein-clearing (TVCV), tobamovirus known easily two model plants, Nicotiana benthamiana, Arabidopsis thaliana. Avoiding restriction digestion, utilized restriction-ligation-independent cloning approach construct infectious cDNA clone TVCV from viral RNA then convert gene adapted Gateway-based recombination transgene insertion. The functionality resulting vector, designated pTVCV-DEST, was validated by autofluorescent reporter following agroinoculation target plant.